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Journal: Cell Regeneration
Article Title: Mammalian mesenchymal stromal cells enhance zebrafish fin regeneration
doi: 10.1186/s13619-025-00273-7
Figure Lengend Snippet: Murine MSCs treated with indomethacin are more susceptible to phagocytosis by activated primary macrophages. A Experimental design: bone marrow-derived monocytes were differentiated into macrophages (Mφ) with M-CSF. Macrophages were then activated with LPS + IFN-γ to induce a pro-inflammatory M1-like phenotype. Activated macrophages were co-cultured in direct contact with control or indomethacin-treated MSCs. Macrophage phenotype was analyzed by flow cytometry (FACS), with all cells gated on the CD45 + CD11b + F4/80 + population, representing differentiated macrophages. B - C Median fluorescence intensity (MFI) of DiD, indicating phagocytosis, was measured in the pro-inflammatory macrophage subpopulations MHC-II + CD86 + ( B ) and MHC-II + CD80 + macrophages ( C ). Representative histograms are shown alongside MFI values. Data are shown as mean ± SD. n = 3 independent experiments. Statistical significance was assessed using one-way ANOVA. *: p < 0.05; **: p < 0.005; ***: p < 0.001; ****: p < 0.0001
Article Snippet: Subsequently, cells were stained with specific
Techniques: Derivative Assay, Cell Culture, Control, Flow Cytometry, Fluorescence